作物花粉自动采集传感器的研制与定标

Development and calibration of crop pollen autoacquisition sensor

摘要:

作物花粉自动采集传感器通过ARM(advanced RISC machines)控制简易机械臂操作采样平台上的载玻片捕获空气中自由扩散的花粉粒子,并将载玻片置于生物显微镜下,通过CMOS(complementary metaloxidesemiconductor)视频传感器将生物显微镜生成的光学图像转换成数字图像,经嵌入式ARM单片机计算处理并存储。利用外场实测资料和标准医用血球计数板,对仪器进行几何尺度标定及载玻片拍摄抽样定标。仪器光学放大倍数F定为328,视场真实面积〖WTB1X〗S〖WTB1〗为1516 mm2,当拍摄抽样数N为50次时,花粉密度相关系数大于95％。当抽样精度一定,花粉浓度大时,可适当减少抽样数目。体积较小的水稻花粉需要拍摄抽样40次,而体积较大的玉米花粉则需要拍摄抽样80次。因系统几何失真引起的光学放大倍数F随显微视场半径变化,使得实测取样面积误差最大可达1375％。

Abstract:

The crop pollen autoacquisition sensor captured crop pollen with a slide on the sampling platform,which was controlled by the simple robotic arm under the ARM(advanced RISC machines) command.Then,the slide was put under biological microscope.By using the CMOS(complementary metaloxidesemiconductor) video sensor,the optical image was changed into digital image,which was to be processed and stored by the ARM.Field experiment data and standard medical blood count board were used to calibrate instrument geometric scale and slide film sampling.The instrument optical magnification F was 328 and the field realspace 〖WTB1X〗S〖WTB1〗 was 1516 mm2.When the sampling number 〖WTB1X〗N〖WTB1〗 was 50,the pollen density correlation coefficient was larger than 95％.With a certain sampling accuracy,when the pollen density was high,sample size could be reduced considerately.The rice pollen whose volume was relatively small needed 40 samples while the bigger corn pollen needed 80 samples.Because of the system geometric distortion,the optical magnification F changed with the radius,which caused the sampling error of the measure area to be as high as 1375％.